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Details of Grant 

EPSRC Reference: EP/D013615/1
Title: A convergent strategy for high efficiency quantitative proteomics
Principal Investigator: Gaskell, Professor S
Other Investigators:
Knowles, Professor J Hubbard, Professor S Kell, Professor DB
Lilley, Professor KS
Researcher Co-Investigators:
Project Partners:
Department: Chemistry
Organisation: University of Manchester, The
Scheme: Standard Research (Pre-FEC)
Starts: 01 October 2005 Ends: 31 March 2009 Value (£): 1,249,267
EPSRC Research Topic Classifications:
Analytical Science Genomics
EPSRC Industrial Sector Classifications:
No relevance to Underpinning Sectors
Related Grants:
EP/D013623/1
Panel History:  
Summary on Grant Application Form
The primary goal of proteomics is to be able to gain the identity and measure the amounts of every protein in the cell in a single experiment. This goal has not yet been achieved, in part because of the sheer molecular complexity of the protein mixture being studied. In this proposal, we aim to streamline the proteomics workflow, by a combination of preparative and analytical strategies. We will develop methods that reduce complexity in a systematic and logical fashion, apply new mass spectrometric techniques to acquire just enough information for identification, and combine these approaches with new methods for absolute quantification. These advances will be transferable to most proteomics laboratories, and are based on fundamental understanding of the behaviour of proteins and peptides derived from proteins in solution and in the mass spectrometer. Because of the novelty of our selective methods, we will need to develop new bioinformatics tools to capitalise on the highly specific and targeted methodologies.
Key Findings
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Summary
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Project URL:  
Further Information:  
Organisation Website: http://www.man.ac.uk