| EPSRC Reference: |
GR/A92774/01 |
| Title: |
STUDY OF THE MECHANISM OF ACTION OF DNA GYRASE AND RELATED PROTEINS USING ATOMIC FORCE MICROSCOPY |
| Principal Investigator: |
Thomson, Dr NH |
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| Department: |
Physics and Astronomy |
| Organisation: |
University of Leeds |
| Scheme: |
Advanced Fellowship (Pre-FEC) |
| Starts: |
27 March 2000 |
Ends: |
26 March 2005 |
Value (£): |
212,104
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| EPSRC Research Topic Classifications: |
| Biological & Medicinal Chem. |
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| EPSRC Industrial Sector Classifications: |
| Healthcare |
Pharmaceuticals and Biotechnology |
| No relevance to Underpinning Sectors |
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Summary on Grant Application Form |
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Atomic force microscopy, a technique which records forces at the nanometre length scale and works analogously to a record player, will be used to probe the interactions of proteins which act on DNA templates through imaging and force measurements. Specifically, the mechanism of action of DNA gyrase, which is responsible for supercoiling DNA in bacteria, and related proteins will be studied. This enzyme is one of a whole number involved in gene expression. Understanding how these proteins work gives the opportunity to develop chemicals (drugs) which inhibit action and therefore act as antibacterial agents. This project will help to elucidate the large amplitudes and long time-scales associated with the action of DNA gyrase and the magnitude of the forces involved. It will increase understanding of gene expression mechanisms, DNA topology and packaging, requirements of design in protein engineering, push the boundaries of nanotechnology (for example patterning of molecules for use as biosensors or biological computers) and give insights into new drugs and their targets. Overall, the project will contribute towards the understanding of the physics of molecular interactions in complex biological systems.
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
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| Date Materialised |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.leeds.ac.uk |