| EPSRC Reference: |
GR/J00311/02 |
| Title: |
X-RAY STRUCTURE OF E.COLI 5-AMINOLAEVULINATE DEHYDRATASE |
| Principal Investigator: |
Cooper, Professor J |
| Other Investigators: |
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| Researcher Co-Investigators: |
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| Project Partners: |
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| Department: |
Centre for Biological Sciences |
| Organisation: |
University of Southampton |
| Scheme: |
Standard Research (Pre-FEC) |
| Starts: |
01 April 1996 |
Ends: |
31 March 1997 |
Value (£): |
59,286
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| EPSRC Research Topic Classifications: |
| Biological & Medicinal Chem. |
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Summary on Grant Application Form |
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The enzyme 5-aminolaevulinate dehydratase catalyses one step in the biosynthesis of essential tetrapyrroles such as haem and chlorophyll. The reaction involves the condensation of two 5-aminolaevulinate residues yielding the pyrrole, porphobilinogen. We have cloned and over-expressed 5-aminolaevulinate debydratase from E.coli and have crystallised the purified enzyme. The crystals diffract to beyond 2.7A resolution and several heavy atom derivatives have been obtained for structure analysis.We request support to allow the structure determination of this fascinating octameric enzyme to be completed. In addition we will analyse active site directed mutants of the enzyme to characterise its reaction mechanism. Our results will have broad implications for understanding how enzymes can stabilise carbanion intermediates.
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Key Findings |
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Potential use in non-academic contexts |
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
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| Date Materialised |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.soton.ac.uk |