| EPSRC Reference: |
GR/J30189/01 |
| Title: |
MICROBIOSENSORS FOR THE INVESTIGATION OF THE ROLE OF SUPEROXIDE RADICALS IN CELL SIGNALLING |
| Principal Investigator: |
Cooper, Professor J |
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| Department: |
Electronics and Electrical Engineering |
| Organisation: |
University of Glasgow |
| Scheme: |
Standard Research (Pre-FEC) |
| Starts: |
01 April 1994 |
Ends: |
31 March 1997 |
Value (£): |
156,981
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| EPSRC Research Topic Classifications: |
| Instrumentation Eng. & Dev. |
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Summary on Grant Application Form |
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To date, it has not been possible to measure superoxide (02-) production directly, and its biological effect has only been inferred by determining the concentrations of presumed by-products of its activity. Recent research by applicants has resulted in the development of a novel, 02- specific amperometric biosensor based upon cytochrome c immobilised at a gold electrode.The programme of research described in this Case for Support aims to further develop our previous work by fabricating arrays of micro-biosensors to measure 0_2- production by individual cells. These sensors will subsequently be used to investigate the role of 02 as a cellular messenger.The research outlined here therefore has four major objectives: (i) the fabrication of micro-biosensors using lithography and their modification with cytochrome c to measure 02-; (ii) the development of suitable target electrodes which will provide dielectrophoretic cues to position individual cells at a specific position at the centre of the concentric micro-biosensors; (iii) the low current measurement of 02- production as a cell signal and, (iv) a quantitative study relating the amount of 02- production with the size of stimuli to cell.
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
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| Date Materialised |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.gla.ac.uk |