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Details of Grant 

EPSRC Reference: GR/J30189/01
Title: MICROBIOSENSORS FOR THE INVESTIGATION OF THE ROLE OF SUPEROXIDE RADICALS IN CELL SIGNALLING
Principal Investigator: Cooper, Professor J
Other Investigators:
McNeil, Professor C Wilkinson, Professor C
Researcher Co-Investigators:
Project Partners:
Department: Electronics and Electrical Engineering
Organisation: University of Glasgow
Scheme: Standard Research (Pre-FEC)
Starts: 01 April 1994 Ends: 31 March 1997 Value (£): 156,981
EPSRC Research Topic Classifications:
Instrumentation Eng. & Dev.
EPSRC Industrial Sector Classifications:
Healthcare
Related Grants:
Panel History:  
Summary on Grant Application Form
To date, it has not been possible to measure superoxide (02-) production directly, and its biological effect has only been inferred by determining the concentrations of presumed by-products of its activity. Recent research by applicants has resulted in the development of a novel, 02- specific amperometric biosensor based upon cytochrome c immobilised at a gold electrode.The programme of research described in this Case for Support aims to further develop our previous work by fabricating arrays of micro-biosensors to measure 0_2- production by individual cells. These sensors will subsequently be used to investigate the role of 02 as a cellular messenger.The research outlined here therefore has four major objectives: (i) the fabrication of micro-biosensors using lithography and their modification with cytochrome c to measure 02-; (ii) the development of suitable target electrodes which will provide dielectrophoretic cues to position individual cells at a specific position at the centre of the concentric micro-biosensors; (iii) the low current measurement of 02- production as a cell signal and, (iv) a quantitative study relating the amount of 02- production with the size of stimuli to cell.
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