| EPSRC Reference: |
GR/J85318/01 |
| Title: |
PROBING RECOGNITION PROCESS USING A MODIFIED ATOMIC FORCE MICROSCOPE |
| Principal Investigator: |
Luckham, Professor PF |
| Other Investigators: |
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| Department: |
Chemical Engineering |
| Organisation: |
Imperial College London |
| Scheme: |
Standard Research (Pre-FEC) |
| Starts: |
01 March 1995 |
Ends: |
31 March 1997 |
Value (£): |
204,030
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Summary on Grant Application Form |
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it is proposed to modify an atomic force microscope to study molecular recognition interactions, in particular the interactions involved in biosystems. Initially a protein or lipid film will be deposited on a flat mica substrate. An antibody or lectin which will interact specifically with the modified substrate will be immobilised on the AFM tip. The interaction forces between these molecules and the surface will then be determined. As an example the interaction between wheat germ agglutinin and lipid head groups containing sialic acid will be studied. Initially particles bearing the active molecules will be attached to the AFM tip. Modified polymer latex particles and colloidal gold commercially available for this purpose. In future we plan to chemically graft the molecules onto AFM tips directly using standard immobilisation chemistry. Thus we shall be able to probe the interactions between individual molecules using AFM technologies. Furthermore the potential exists for extending this technique to investigate cell surface interactions.
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
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| Date Materialised |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.imperial.ac.uk |