| EPSRC Reference: |
GR/L72084/01 |
| Title: |
ENVIRONMENTAL BIOGENICS USING SENSOR-REGULATED GENES FOR IN SITU ASSESSMENT OF POLLUTANTS IN SOIL |
| Principal Investigator: |
Edwards, Professor C |
| Other Investigators: |
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| Researcher Co-Investigators: |
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| Project Partners: |
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| Department: |
Sch of Biological Sciences |
| Organisation: |
University of Liverpool |
| Scheme: |
Standard Research (Pre-FEC) |
| Starts: |
01 February 1998 |
Ends: |
31 March 2001 |
Value (£): |
154,254
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| EPSRC Research Topic Classifications: |
| Assess/Remediate Contamination |
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Summary on Grant Application Form |
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Bacterial whole cell sensors of pollutants will be designed that exploit molecular switches called promoters, which activate specific cellular activities in response to an extracellular stimulus. Different promoters will be genetically-linked to a novel gene, the product of which will act as a reporter of promoter activation. Therefore the presence of an external stimulus, which in this programme will be mainly aromatic pollutants, can be detected by the reporter gene. This gene (din) encodes for DNA invertase which inverts defined segments of DNA permanently resulting in a region of changed sequence. This change will be detected by the polymerase chain reaction and a PCR-based assay of din expression optimised. These bacteria will function as whole cell biosensors and once a reproducible assay has been developed they will be used to audit pollution in environment samples. It is hoped to produce novel cell biosensors for environmental monitoring and detection of chemical pollutants.
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
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| Date Materialised |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.liv.ac.uk |