| EPSRC Reference: |
GR/M45696/01 |
| Title: |
PROBING DNA INTERACTIONS AT COLLOIDAL SURFACES & WITH METAL COMPLEXES IN VITRO |
| Principal Investigator: |
McGarvey, Professor J |
| Other Investigators: |
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| Researcher Co-Investigators: |
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| Project Partners: |
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| Department: |
Sch of Chemistry and Chemical Eng |
| Organisation: |
Queen's University of Belfast |
| Scheme: |
Standard Research (Pre-FEC) |
| Starts: |
01 August 1999 |
Ends: |
31 May 2003 |
Value (£): |
252,583
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| EPSRC Research Topic Classifications: |
| Complex fluids & soft solids |
Surfaces & Interfaces |
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| EPSRC Industrial Sector Classifications: |
| Chemicals |
Environment |
| Food and Drink |
Healthcare |
| Pharmaceuticals and Biotechnology |
No relevance to Underpinning Sectors |
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Summary on Grant Application Form |
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Surface-enhanced Raman spectroscopy (SERS) will be employed to investigate the molecular recognition of single-stranded(ss) DNA (appropriately labelled) at colloidal surfaces. Metal liquid-like films (previously proven at QUB to be effective surfaces for SERS) will be used as one type of active substrate upon which the specific sequence of target ss-DNA can be determined. As a novel slant to this part of the research, DNA hybridization will be used to bring abut controlled aggregation of oligonucelotide-loaded colloidal metal particles, providing an 'in situ' means of increasing the surface-enhanced scattering intensity and hence of the sensitivity of detection of an incorporated, DNA-tethered label.Time-resolved Raman studies, at QUB and the Central Laser Facility, will explore how modifications to intercalating ligands of metal complexes may influence the extent of excited state interaction with the relevant form of DNA (e.g. ss or ds). Extension to DNA-intercalating metal complexes proposed to participate in photo-induced electron transfer along ds DNA will be undertaken. The utility of such phenomena for the detection of DNA sequence through hybridisation will also be explored. Coherent anti-Stokes Raman spectroscopy will be studied as a more refined structural probe of intractions of DNA with ground and excited states of metal complex intercalators.
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
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| Date Materialised |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.qub.ac.uk |