| EPSRC Reference: |
GR/M63744/01 |
| Title: |
ENZYME-BASED QUARTZ CRYSTAL BIOSENSORS FOR ANALYTES OF BIOMEDICAL SIGNIFICANCE |
| Principal Investigator: |
Reddy, Professor S |
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| Project Partners: |
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| Department: |
Health and Medical Sciences |
| Organisation: |
University of Surrey |
| Scheme: |
Standard Research (Pre-FEC) |
| Starts: |
18 October 1999 |
Ends: |
17 October 2002 |
Value (£): |
52,953
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| EPSRC Research Topic Classifications: |
| Materials Characterisation |
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| EPSRC Industrial Sector Classifications: |
| Chemicals |
No relevance to Underpinning Sectors |
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Summary on Grant Application Form |
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When a resonating AT-cut quartz crystal is interfaced with a test liquid, the propagating shear waves are readily absorbed within a defined region above the crystal surface. The quartz crystal is able to detect viscoelastic changes within this sheer wave sensing region. Strategies will be devised to induce the formation of analyte-specific viscoelastic layers using enzyme reactions. The family of enzymes to be exploited are the oxidases. The enzyme will be chemically immobilised and retained between permselective membranes above the crystal surface. Hydrogen peroxide is the key product which, in the presence of a second enzyme (peroxidase), will catalytically oxidise redox active arylamines to form azo and indophenol compounds. The sparingly soluble nature of some of these products can be used to exploit he sensing capability of the quartz crystal. Accordingly, biosensors (operating in both buffer solution and real biological samples) will be realised for cholesterol and creatinine. Packing efficiency of the deposited multi-layers will be studied using SEM. Impedance analysis will provide a clearer picture as to the methods of improving sensor response charateristics. The potential problem of viscotic interferance from proteins and cellular material will be addressed using membrane based solute- gating stratergies.
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
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| Date Materialised |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.surrey.ac.uk |