| EPSRC Reference: |
GR/N18772/01 |
| Title: |
POLYDYES: TARGETED MOLECULAR TRANSDUCERS FOR PHARMACEUTICAL SCREENING |
| Principal Investigator: |
Slater, Professor N |
| Other Investigators: |
|
| Researcher Co-Investigators: |
|
| Project Partners: |
|
| Department: |
Chemical Engineering and Biotechnology |
| Organisation: |
University of Cambridge |
| Scheme: |
Standard Research (Pre-FEC) |
| Starts: |
01 July 2000 |
Ends: |
30 June 2003 |
Value (£): |
195,856
|
| EPSRC Research Topic Classifications: |
| Bioprocess Engineering |
Chemical Biology |
| Drug Formulation & Delivery |
|
|
| EPSRC Industrial Sector Classifications: |
| Pharmaceuticals and Biotechnology |
No relevance to Underpinning Sectors |
|
| Related Grants: |
|
| Panel History: |
|
|
Summary on Grant Application Form |
|
This proposal addresses the design and synthesis of polymeric fluorescent probes that facilitate highly sensitive optical detection of a range of biochemical and physiological events by exploiting fundamental physical principles of polymer photophysics. In preliminary work we have shown that the incorporation of Cy-3 dyes in the backbone of certain amphiphilic hypercoiling polymers can provide environmentally sensitive fluorescence amplification through the orientation of dyes on the polymer. We now envisage synthetic polymeric probes that can be used with emerging CCD imaging technology to permit the highly sensitive measurement of fluorescence from intensified cell-based assays. Initial pH responsive probes will be adapted to respond to other events that lead to the modulation of charge on specific polymer-pendant ligands. Assays employing these probes will be more flexible and sensitive than others that rely upon either radioactivity, monomeric fluorescent tags or enzyme-induced fluorescence to provide lower optical output. As a secondary objective we envisage variants of these probes that would exploit the known transmission properties of near-infrared optical signals through tissues to permit non-invasive in-vivo imaging reagents that can be cheaply and conveniently applied in clinics.EngProg\
|
|
Key Findings |
|
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
|
|
Potential use in non-academic contexts |
|
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
|
|
Impacts |
|
| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
|
| Date Materialised |
|
|
|
|
Sectors submitted by the Researcher |
|
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
|
| Project URL: |
|
| Further Information: |
|
| Organisation Website: |
http://www.cam.ac.uk |