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Details of Grant 

EPSRC Reference: GR/N19052/01
Title: MULTIPHOTON,X-RAY&UV MICRO-IRRADIATION OF SUB-CELLULAR COMPONENTS TO TRACK INTRA-CELLULAR DYNAMICS
Principal Investigator: Wharton, Professor C
Other Investigators:
Botchway, Professor SW Hirst, Dr G Chipman, Professor JK
Researcher Co-Investigators:
Dr R Meldrum
Project Partners:
Stanford University
Department: Sch of Biosciences
Organisation: University of Birmingham
Scheme: Standard Research (Pre-FEC)
Starts: 01 April 2000 Ends: 30 September 2002 Value (£): 144,137
EPSRC Research Topic Classifications:
Cells Lasers & Optics
EPSRC Industrial Sector Classifications:
Pharmaceuticals and Biotechnology No relevance to Underpinning Sectors
Related Grants:
Panel History:  
Summary on Grant Application Form
The central aim in modern biochemistry is to observe, as directly as possible, enzymatic processes in living cells. To achieve this, methods need to be developed for initiating reactions in very small subcellular volumes. Techniques of deep UV and multiphoton micro-irradation which will allow discrete areas of a cell nucleus to be irradiated offer a unique opportunity to study the dynamic responses of cell proteins to the induction of DNA damage. Once this is achieved the dynamic response, in terms of position, rate, topology and kinetics of DNA and nuclear proteins to UV-induced damage can be studied.Development of precision micro-masking and spectral tailoring of soft x-rays from a pulsed laser plasma source will allow the dynamics of intercellular communication between damaged and undamaged cells to be observed.This multidisciplinary project, at the boundary between the physical and life sciences, will provide state-of-the-art technology to underpin fundamental research in the areas of biochemistry and biological cell dynamics.
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Further Information:  
Organisation Website: http://www.bham.ac.uk