| EPSRC Reference: |
GR/N20034/01 |
| Title: |
NOVEL OPTICAL MANIPULATION TECHNIQUES AND THEIR APPLICATION TO CHROMATID BREAK STUDIES |
| Principal Investigator: |
Dholakia, Professor K |
| Other Investigators: |
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| Researcher Co-Investigators: |
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| Project Partners: |
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| Department: |
Physics and Astronomy |
| Organisation: |
University of St Andrews |
| Scheme: |
Standard Research (Pre-FEC) |
| Starts: |
01 September 2000 |
Ends: |
28 February 2003 |
Value (£): |
172,059
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| No relevance to Underpinning Sectors |
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Summary on Grant Application Form |
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The programme described will have two major areas that overlap strongly between the Schools of Physics and Biology at the University of St Andrews.a. The development and characterisation of an optical tweezer arrangement using Laguerre-Gaussian light beams. The first full 3D evaluation of these light beams should lead to a tweezing system that requires reduced input power and thus limits any optical damage that may occur to the trapped sample. Additionally characterisation of particle manipulation will be performed using interfering light beams for enhanced translational and rotational control of trapped objects in the tweezer. These studies will be directly linked to the fixed metaphase chromatid studies in part (b).b. The optical microdissection and subsequent manipulation of chromosome fragments. Using a 532nm laser, small chromatid fragments will be generated with precision. Optical tweezers will then be used (utilising work from part (a)) to move the fragments to a suitable well for access by a micropipette for amplification using Polymerased Chain Reaction.EngProg\
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
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| Date Materialised |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.st-and.ac.uk |