| EPSRC Reference: |
GR/N29549/01 |
| Title: |
STRUCTURAL ELUCIDATION OF BIOMATERIALS BY VERY HIGH SPEED MAS PROTON AND OXYGEN-17 NMR AT ULTRAHIGH FIELDS |
| Principal Investigator: |
Dupree, Professor R |
| Other Investigators: |
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| Project Partners: |
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| Department: |
Physics |
| Organisation: |
University of Warwick |
| Scheme: |
Standard Research (Pre-FEC) |
| Starts: |
23 October 2000 |
Ends: |
22 October 2002 |
Value (£): |
124,410
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| EPSRC Research Topic Classifications: |
| Biological & Medicinal Chem. |
Chemical Biology |
| Chemical Structure |
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| EPSRC Industrial Sector Classifications: |
| Chemicals |
Healthcare |
| Pharmaceuticals and Biotechnology |
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Summary on Grant Application Form |
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The structural resolution of large (Mr(eff) ~ Mda when in bilayers) membrane proteins is at the forefront of structural biology, but is not generally possible with diffraction techniques or liquid state NMR due to technical hurdles caused by crystallisation problems and their large effective size, respectively. Solid-state NMR is making an impact in the area, through exploiting carbon-13, nitrogen-15 specific labelling. However, the information-rich proton spectrum has poor resolution due to strong, through-space, homonuclear dipolar couplings. These couplings (D ~ 30-100kHz) can be removed by spinning the sample at high speeds (omega(r) >D) around the 'magic angle' of 54.7deg removing anisotropic interactions (which are {3cos(sq)theta-1} dependent), and in high (>600MHz for proton) magnetic fields. NMR probes capable of spinning at 50kHz will be developed for use at 600MHz and 800MHz and high-resolution proton spectra biologically important materials in the solid state will be obtained for the first time. Novel NMR experiments will be developed to take advantage of the information available in the spectra and the techniques will be initially demonstrated in a structural study of a biologically and pharmacologically significant biomolecule.
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
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| Date Materialised |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.warwick.ac.uk |