| EPSRC Reference: |
GR/N63161/01 |
| Title: |
A NEW EYE FOR SYCLOPS - A SPECTROSCOPY UPGRADE FOR THE DARESBURY CONFOCAL MICROSCOPE |
| Principal Investigator: |
ODonnell, Professor K |
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| Project Partners: |
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| Department: |
Physics |
| Organisation: |
University of Strathclyde |
| Scheme: |
Standard Research (Pre-FEC) |
| Starts: |
16 October 2000 |
Ends: |
15 July 2002 |
Value (£): |
30,570
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| EPSRC Research Topic Classifications: |
| Biological & Medicinal Chem. |
Cells |
| Chemical Structure |
Materials Characterisation |
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| EPSRC Industrial Sector Classifications: |
| Chemicals |
Electronics |
| Pharmaceuticals and Biotechnology |
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Summary on Grant Application Form |
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We propose to upgrade the Daresbury Laboratories confocal microscope SYCLOPS by enhancing its spectroscopic capabilities. The microscope features confocal illumination and detection optics arranged on an optical bench for flexibility of application, and an attached scanning mirror head, objective lens mount, and cooled sample stage. Laser sources are commonly used for high resolution fluorescence imaging, while synchrotron radiation provides a convenient pulsed source for fluorescence lifetime spectroscopy, in addition to allowing excitation at high photon energies. In the present set-up, crude spectral sorting of the fluorescence by interference filters allows the acquisition of a series of quasi-monochromatic images. By replacing the photomultiplier tube that detecs fluorescence with CCD/spectrometer combination, we will greatly enhance the spectroscopic capability of the instrument. The microscope will be capable of performing spectroscopy of spatially selected objects as small as the diffraction limit allows. It will also be able to produce 'spectrum images' that combine the advantages of high spatial and spectral resolution. The unique capabilities of the microscope will be tested on several different kinds of samples in an extension of a current materials portfolio which embraces semiconductor physics, organic chemistry, cell biology and the semiconductor/neuron interface.
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
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| Date Materialised |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.strath.ac.uk |