| EPSRC Reference: |
GR/R67439/01 |
| Title: |
Peptide activity in supported phospholipid layers using electrochemical and optical techniques |
| Principal Investigator: |
Nelson, Professor LA |
| Other Investigators: |
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| Researcher Co-Investigators: |
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| Project Partners: |
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| Department: |
Sch of Chemistry |
| Organisation: |
University of Leeds |
| Scheme: |
Fast Stream |
| Starts: |
01 February 2002 |
Ends: |
31 January 2005 |
Value (£): |
63,120
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| EPSRC Research Topic Classifications: |
| Biological & Medicinal Chem. |
Cells |
| Chemical Biology |
Surfaces & Interfaces |
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| EPSRC Industrial Sector Classifications: |
| Aerospace, Defence and Marine |
Chemicals |
| Pharmaceuticals and Biotechnology |
Water |
| No relevance to Underpinning Sectors |
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| Related Grants: |
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| Panel History: |
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Summary on Grant Application Form |
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Phospholipids and proteins are the main constituents of biological membranes. An understanding of the interactions between these biomolecules is essential for a greater insight into membrane structure and function . Peptides are the constituent chains of proteins and this proposed work will study phospholipid-peptide interactions in membrane models. Particular attention will be paid to investigating the interactions between the peptide and the phospholipid at the lipid-water interface. The proposal will use novel electrochemical techniques of fast pulsing and impedance to study the processes within the layers deposited on electrode surfaces. Langmuir-Blodgett methods will be used to deposit the phospholipid-peptide layers on to solid conducting supports. Optical techniques of Atomic Force and Fluorescence Microscopy will be applied to further understand the properties of the peptide in the solid supported phospholipid layers and the effect of voltage field thereon. The model peptide gramicidin-A and its derivatives will be initially studied. Later work will extend the investigations to other biologically- active peptides and short chain peptides custom designed in the host laboratory. The work has applications to developing systems for the sensing of biologically active peptides and other compounds in aqueous systems.
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
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| Date Materialised |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.leeds.ac.uk |