| EPSRC Reference: |
GR/R81916/01 |
| Title: |
Second harmonic imaging of cell membrane structures |
| Principal Investigator: |
O'Shea, Professor P |
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| Researcher Co-Investigators: |
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| Project Partners: |
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| Department: |
Sch of Biomedical Sciences |
| Organisation: |
University of Nottingham |
| Scheme: |
Postdoctoral Mobility PreFEC |
| Starts: |
01 March 2002 |
Ends: |
28 February 2003 |
Value (£): |
70,414
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| EPSRC Research Topic Classifications: |
| Cells |
Chemical Biology |
| Complex fluids & soft solids |
Surfaces & Interfaces |
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| EPSRC Industrial Sector Classifications: |
| No relevance to Underpinning Sectors |
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Summary on Grant Application Form |
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The purpose of this work is to allow the named postdoctoral Research Assistant, NBES, to transfer knowledge and technology between the Optical Engineering Group (OEG) of the School of Electrical and Electronic Engineering and the Cell Biophysics Group (CBG) of the School of Biomedical Sciences under the Postdoctoral mobility scheme, both schools being parts of the University of Nottingham.The project will demonstrate the feasibility of imaging cell membranes using the novel second harmonic imaging modes of TIR, SP and SP with layered media. They will be implemented by reconfiguring an existing short pulse, multi-photon excitation confocal microscope. The results obtained with these modes will be compared with those obtained using established methods. This will include images captured using a wide field confocal microscope developed by NBES in the OEG. The research will establish the difference in the structural and functional information provided by each of the novel and established modes. A model relating the response of each imaging mode to the susceptibilities of a sample will be initiated.The binding of macromolecules such as Alzheimers peptides and serum albumin with artificial membrane systems has been chosen as a subject for the various imaging modes as we have already shown that they exhibit substantial structural changes on binding to membranes.
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
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| Date Materialised |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.nottingham.ac.uk |