| EPSRC Reference: |
GR/S04888/01 |
| Title: |
Surface enhanced resonance Raman coded beads : New technology for ultra-sensitivce analysis of multiple events |
| Principal Investigator: |
Smith, Professor WE |
| Other Investigators: |
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| Project Partners: |
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| Department: |
Pure and Applied Chemistry |
| Organisation: |
University of Strathclyde |
| Scheme: |
Standard Research (Pre-FEC) |
| Starts: |
01 May 2003 |
Ends: |
31 October 2006 |
Value (£): |
303,661
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| EPSRC Research Topic Classifications: |
| Biological & Medicinal Chem. |
Complex fluids & soft solids |
| Materials Characterisation |
Materials Processing |
| Particle Technology |
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| EPSRC Industrial Sector Classifications: |
| No relevance to Underpinning Sectors |
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Summary on Grant Application Form |
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Surface enhanced resonance Raman scattering (SERRS) gives sharp molecularly specific spectra from dyes adsorbed onto rough surfaces of silver and gold. There is much greater multiplexing capability and at least equivalent sensitivity to fluorescence. Development of functionalised polymer beads containing dye-labelled, pre-aggregated and encapsulated silver nanoparticles is proposed. The silver particles are protected from the environment and immobilised by the polymer so that the SERRS, which depends on the degree of aggregation, is constant and the bead is biologically inert. The analytical event is achieved separately by covalently attaching the detection agent to the functionalised polymer surface. Key advantages are that the beads give very intense, stable SERRS for simple, sensitive identification of a specific bead and the sharp signals provide a huge selective labelling capability which is unrivalled by any other technique and which will allow discrimination of one bead in situ in a very large number of differently labelled beads. Fields in which this multiplexing capability will give a new dimension include DNA detection, proteomics, cell and antibody recognition, drug discovery and combinatorial chemistry. To provide focus for a completely new technology, only the use for DNA recognition and for polypeptide identification is developed.
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
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| Date Materialised |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.strath.ac.uk |