| EPSRC Reference: |
GR/S16621/02 |
| Title: |
Development of Thiol Specific Chemical Sensors for the Near Patient Monitoring of Oxidative Stress |
| Principal Investigator: |
Davis, Professor J |
| Other Investigators: |
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| Researcher Co-Investigators: |
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| Project Partners: |
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| Department: |
School of Science & Technology |
| Organisation: |
Nottingham Trent University |
| Scheme: |
First Grant Scheme Pre-FEC |
| Starts: |
01 October 2005 |
Ends: |
30 September 2007 |
Value (£): |
42,998
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| EPSRC Research Topic Classifications: |
| Med.Instrument.Device& Equip. |
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Summary on Grant Application Form |
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The project seeks to develop a new electrochemical sensor that can aid the point of care diagnosis and management of clinical conditions brought about by oxidative stress processes. A novel approach to the selective detection and measurement of thiol biomarkers (principally cysteine and glutathione) within physiological fluids would be developed. The methodology would be based upon the selective reaction of thiol species with a quinone whereupon the electrochemical detection of the resulting conjugate would provide the analytical signal. A range of quinoid indicators would be investigated and their electrochemical properties assessed such that the operating characteristics of the sensor could be optimised to preclude interference from other electroactive constituents (typically ascorbate). A number of electrode designs/prototypes would be produced whereby the indicating species is immobilised to provide a reagentless device suitable for operation by attendent biomedical staff. Validation of the sensing strategy would be performed through comparison with traditional methods of thiol analysis (HPLC/Ellmans) and through inter-laboratory checks. The efficacy of the approach to near patient monitoring would be demonstrated through conducting preliminary clinical trials on whole blood samples extracted from diabetic patients presenting complications characteristic of oxidative stress.
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
| Summary |
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| Date Materialised |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.ntu.ac.uk |