| EPSRC Reference: |
GR/S82800/01 |
| Title: |
KF Reardon Visiting Fellowship: Shotgun gel free proteomics of cyanobacteria for renewable energy and environmental applications |
| Principal Investigator: |
Wright, Professor P |
| Other Investigators: |
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| Department: |
Chemical & Biological Engineering |
| Organisation: |
University of Sheffield |
| Scheme: |
Standard Research (Pre-FEC) |
| Starts: |
04 August 2004 |
Ends: |
03 July 2005 |
Value (£): |
61,422
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| EPSRC Research Topic Classifications: |
| Bioprocess Engineering |
Design of Process systems |
| Genomics |
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| EPSRC Industrial Sector Classifications: |
| Pharmaceuticals and Biotechnology |
Energy |
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Summary on Grant Application Form |
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We seek to transfer techniques from leading edge post genomics endeavours from US biochemical engineering to both biochemical engineering and bioscience groups within the UK, facilitated through a 6 month visiting fellowship for Professor KF Reardon of Colorado State University. Proteomics (full set of proteins within the cell) lags behind other post-genomic technologies, yet has enormous future biotechnological potential as it directly relates to what the cells are actually doing . Two-dimensional gel electrophoresis (2DE) is conventionally used for proteomic analysis, yet suffers from many disadvantages such as low throughput (cumbersome) and difficulty in resolving potentially important proteins such as low abundance or membrane proteins. Here, we will develop cutting edge technologies, to supercede 2DE for cyanobacterial proteomics, especially multi-dimensional liquid chromatography, to resolve these difficult proteins and quantify them using (primarily) isotope coded affinity tag analysis. This will be benchmarked against 2DE to assess reproducibility and advantages and disadvantages. Cyanobacteria are chosen here as they are found to inhabit terrestrial and aquatic habitats (thus are environmentally important), and produce a myriad of potentially industrially important chemicals. Furthermore, only limited proteomics studies (2DE) have been carried out. The visiting fellowship will allow for two way information exchange between the direct researchers, as well as more widely across the UK, via a wider visiting dissemination programme.
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Key Findings |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Potential use in non-academic contexts |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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Impacts |
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| Description |
This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk |
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Sectors submitted by the Researcher |
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This information can now be found on Gateway to Research (GtR) http://gtr.rcuk.ac.uk
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| Project URL: |
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| Further Information: |
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| Organisation Website: |
http://www.shef.ac.uk |